4EBP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

4EBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

55

CloneNo.

2C3F3

Reviews (2)

Cat no : 60246-1-Ig

Synonyms

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

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Validation Data Gallery

• 

  WB analysis of K562 using 60246-1-Ig

  WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells.

• 

  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60246-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 60246-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 60246-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60246-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60246-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562
• Positive IHC detected in: human pancreas cancer tissue, mouse liver tissue, rat liver tissue, rat pancreas tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

60246-1-Ig targets 4EBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Cited Reactivity: human, chicken
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: 4EBP1 fusion protein Ag18985
• Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Calculated Molecular Weight: 118 aa, 12 kDa
• Observed Molecular Weight: 15-24 kDa
• GenBank Accession Number: BC004459
• Gene Symbol: EIF4EBP1
• Gene ID (NCBI): 1978
• RRID: AB_2881368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

Publications

Species	Application	Title
	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	IHC	Mol Cancer; The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.; Authors - Liang-Yan Chen
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
	WB,IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (03-02-2021)

10ug of HEK293 lysate resolved on 4-20% gradient acrylamide gel. Membrane blocked (5% BSA) then incubated overnight with antibody (1:2,000). Incubated with secondary HRP antibody for visulization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293

4EBP1 Antibody Western Blot validation (1:2000 dilution) in HEK293 (Cat no:60246-1-Ig)

FH

Yifei (Verified Customer) (11-01-2017)

It works well for Western blot

• Applications: Western Blot

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