Anticorps Monoclonal anti-PI3 Kinase p85 Alpha

• Phare
• Validé par KD/KO

PI3 Kinase p85 Alpha Monoclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris et plus (2)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

138

CloneNo.

4G3C11

N° de cat : 60225-1-Ig

Synonymes

GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-kinase p85α, PI3 Kinase p85 Alpha, PI3 kinase subunit p85 alpha, PI3K p85(alpha), PI3K regulatory subunit alpha, PIK3R1

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Galerie de données de validation

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  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis of rat skeletal muscle using 60225-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 60225-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60225-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60225-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60225-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60225-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60225-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60225-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60225-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 60225-1-Ig

  IP Result of anti-PI3K p85(alpha) (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug.

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules Jurkat, cellules Neuro-2a, cellules PC-12, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal, tissu de muscle squelettique de porc, tissu de muscle squelettique de rat
• Résultats positifs en IP: cellules RAW 264.7
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu cérébral de souris, tissu de cancer de la prostate humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 134 publications below
• IHC: See 8 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60225-1-Ig cible PI3 Kinase p85 Alpha dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p85 Alpha Protéine recombinante Ag2344
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC030815
• Symbole du gène: PIK3R1
• Identification du gène (NCBI): 5295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

Publications

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human	WB	Autophagy; The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis.; Authors - Shulan Pi
human	WB	Oncogene; MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.; Authors - Ran-Ran Ma
mouse	WB	Phytother Res; 11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2 /ERβ signaling pathways; Authors - Yan Lyu
rat	WB	Environ Pollut; Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.; Authors - Rundong Liu
mouse	WB	Arterioscler Thromb Vasc Biol; Short-Term Exposure to Waterpipe/Hookah Smoke Triggers a Hyperactive Platelet Activation State and Increases the Risk of Thrombogenesis.; Authors - Ahmed B Alarabi

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