PI3 Kinase p110 Gamma Polyclonal antibody

PI3 Kinase p110 Gamma Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20662-1-AP

Synonyms

p120 PI3K, PI3 Kinase p110 Gamma, PI3 kinase subunit gamma, PI3CG, PI3K, PI3K gamma, PI3K p110(gamma), PI3Kgamma, PIK3, PIK3CG, PtdIns 3 kinase subunit gamma

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Validation Data Gallery

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  WB analysis using 20662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 20662-1-AP

  IP result of anti-PI3 Kinase p110 Gamma (IP:20662-1-AP, 5ug; Detection:20662-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 20662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of mouse skeletal muscle using 20662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20662-1-AP (PI3 Kinase p110 Gamma antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with .

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20662-1-AP targets PI3 Kinase p110 Gamma in WB, IP, IHC, ELISA applications and shows reactivity with Rat, mouse samples.

• Tested Reactivity: Rat, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, catalytic, gamma polypeptide
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_002649
• Gene Symbol: PIK3CG
• Gene ID (NCBI): 5294
• RRID: AB_2878720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Am J Physiol Endocrinol Metab; Frequent and mild scrotal heat stress in mice epigenetically alters glucose metabolism in the male offspring.; Authors - Xiaoyan Wan
mouse	WB	Am J Pathol; Suppression of Overactive Insulin-Like Growth Factor 1 Attenuates Trauma-Induced Heterotopic Ossification in Mice; Authors - Dong Mao