PI3 Kinase p110 Alpha Polyclonal antibody

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 20583-1-AP

Synonyms

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

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Validation Data Gallery

• 

  WB analysis of HepG2 using 20583-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20583-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20583-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20583-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20583-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 20583-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20583-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, COLO 320 cells, HeLa cells, A431 cells, mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 3 publications below

Product Information

20583-1-AP targets PI3 Kinase p110 Alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_006218
• Gene Symbol: PIK3CA
• Gene ID (NCBI): 5290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.; Authors - H Zhang
human	WB	Oncotarget; Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.; Authors - Xiao-Qing Wang
human	IHC	J Cancer; Vasculogenic Mimicry in Prostate Cancer: The Roles of EphA2 and PI3K.; Authors - Hua Wang
mouse	WB	Mol Med Rep; Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells; Authors - Xinyan Pan
	WB	Dev Comp Immunol; Characterization, functional and signaling elucidation of pigeon (Columba livia) interferon-α: Knockdown p53 negatively modulates antiviral response.; Authors - Siwen Li
		Biomed Pharmacother; Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.; Authors - Jing-Jing Mou

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