GRB2 Polyclonal antibody

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GRB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10254-2-AP Copied!

Publications (27)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Growth factor receptor-bound protein 2, Grb33, EGFRBP GRB2, ASH

• 

  WB analysis of HEK-293 using 10254-2-AP

  WB result of GRB2 antibody (10254-2-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRB2 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 10254-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 10254-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 10254-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat spleen using 10254-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10254-2-AP (GRB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 10254-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10254-2-AP (GRB2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 10254-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10254-2-AP (GRB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, rat brain tissue, rat spleen tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10254-2-AP targets GRB2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0395

  Product name: Recombinant human GRB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-214 aa of BC000631

  Sequence: MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVN

• Full Name: growth factor receptor-bound protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC000631
• Gene Symbol: GRB2
• Gene ID (NCBI): 2885
• RRID: AB_2263577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62993
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Publications

What published studies show

Anti-GRB2 antibody (Cat# 10254-2-AP) has 27 total citations published in journals including Advanced Science, Science Advances, Neuro-Oncology, Cancer Letters, Oncogene, Journal of Experimental & Clinical Cancer Research, and Biochimica et Biophysica Acta. Research fields encompass oncology (hepatocellular carcinoma, breast cancer, glioblastoma, lung and pancreatic cancer), RAS/MAPK and PI3K/AKT signaling, bone metabolism, hepatic fibrosis, wound healing, preeclampsia, and infectious disease mechanisms.

Automatically generated from the 27 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	J Exp Clin Cancer Res; Bufalin inhibits hepatocellular carcinoma progression by blocking EGFR-mediated RAS-RAF-MEK-ERK pathway activation; Authors - Jingwen Liu
		Adv Sci (Weinh); Amuc_1473 Links Gut Microbes to Skeletal Homeostasis and Counteracts Multifactorial Osteoporosis.; Authors - Shan-Shan Rao
human	IF	Adv Sci (Weinh); Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer; Authors - Xinwen Wang
human	IF	Sci Adv; HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis; Authors - Qiwei Jiang
human	IHC	Neuro Oncol; ROR1 facilitates glioblastoma growth via stabilizing GRB2 to promote c-Fos expression in glioma stem cells; Authors - Hongtao Zhu
		Cancer Lett; Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.; Authors - Xinjing Wang