INSR Polyclonal antibody

INSR Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, IP, CoIP, Dot blot, ELISA

Conjugate

Unconjugated

Publications

29

Reviews (3)

Cat no : 20433-1-AP

Synonyms

CD220, HHF5, INSR, IR

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Validation Data Gallery

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  WB analysis of HEK-293 using 20433-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20433-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 20433-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20433-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20433-1-AP (INSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20433-1-AP

  IP result of anti-INSR (IP:20433-1-AP, 4ug; Detection:20433-1-AP 1:500) with HeLa cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 27 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

20433-1-AP targets INSR in WB, IHC, IF, IP, CoIP, Dot blot, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, zebrafish, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INSR fusion protein Ag13922
• Full Name: INSR
• Calculated Molecular Weight: 1382 aa, 156 kDa
• Observed Molecular Weight: 190 kDa, 135 kDa, 95 kDa
• GenBank Accession Number: BC117172
• Gene Symbol: INSR
• Gene ID (NCBI): 3643
• RRID: AB_10646469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The biological effects of INS are mediated by a membrane-spanning cell surface receptor that has been shown to consist of disulfide-linked alpha-subunits (135 kDa) and beta-subunits (95 kDa) that are cleaved products of a common precursor. The binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.

Publications

Species	Application	Title
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
rat	WB	Food Funct; l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways; Authors - Ling Lin
human	WB	Br J Surg; MiR-128 regulation of glucose metabolism and cell proliferation in triple-negative breast cancer.; Authors - M Xiao
human	WB	FASEB J; Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.; Authors - Tong Xu
mouse	WB,IHC	Sci Rep; NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.; Authors - Shu Yang
mouse	WB	J Mol Med (Berl); Metabolomics reveals that chronic restraint stress alleviates carbon tetrachloride-induced hepatic fibrosis through the INSR/PI3K/AKT/AMPK pathway; Authors - Shanshan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (05-29-2023)

It works well in human white adipocytes, we get strong signals

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: adipocytes

FH

Chiara (Verified Customer) (10-04-2021)

Works well but three bands

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell

FH

Boyan (Verified Customer) (06-06-2021)

This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.

• Applications: Western Blot