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PI3 Kinase p110 Beta Polyclonal antibody
PI3 Kinase p110 Beta Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 20584-1-AP
|Positive WB detected in||rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20584-1-AP targets PI3 Kinase p110 Beta in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse, rat, chicken, rabbit, fish, zebrafish, pig, canine, Megalobrama amblycephala|
|Host / Isotype||Rabbit / IgG|
|Full Name||phosphoinositide-3-kinase, catalytic, beta polypeptide|
|Calculated molecular weight||123 kDa|
|Observed molecular weight||110-130 kDa|
|GenBank accession number||NM_006219|
|Gene ID (NCBI)||5291|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.
|Product Specific Protocols|
|WB protocol for PI3 Kinase p110 Beta antibody 20584-1-AP||Download protocol|
|IHC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP||Download protocol|
|IP protocol for PI3 Kinase p110 Beta antibody 20584-1-AP||Download protocol|
|FC protocol for PI3 Kinase p110 Beta antibody 20584-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Macarena Lucia (Verified Customer) (10-17-2022)