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PI3 Kinase p85 Alpha Monoclonal antibody

PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, pig, rat, rabbit and More (2)







Cat no : 60225-1-Ig


GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-kinase p85α, PI3 Kinase p85 Alpha, PI3 kinase subunit p85 alpha, PI3K p85(alpha), PI3K regulatory subunit alpha, PIK3R1

Tested Applications

Positive WB detected inHEK-293 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, pig brain tissue, A549 cells, Jurkat cells, Neuro-2a cells, PC-12 cells, rat skeletal muscle tissue, rabbit brain tissue, pig skeletal muscle tissue
Positive IP detected inRAW 264.7 cells
Positive IHC detected inmouse lung tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60225-1-Ig targets PI3 Kinase p85 Alpha in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, pig, rat, rabbit samples.

Tested Reactivity human, mouse, pig, rat, rabbit
Cited Reactivityhuman, mouse, rat, pig, canine, bovine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Alpha fusion protein Ag2344
Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC030815
Gene Symbol PIK3R1
Gene ID (NCBI) 5295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.


Product Specific Protocols
WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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