PI3 Kinase p85 Alpha Monoclonal antibody

• Featured Product
• KD/KO Validated

PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

183

CloneNo.

4G3C11

Cat no : 60225-1-Ig

Synonyms

P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

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Validation Data Gallery

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  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 60225-1-Ig

  WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60225-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 60225-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60225-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 60225-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 60225-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60225-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60225-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60225-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60225-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60225-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 60225-1-Ig

  IP result of anti-PI3 Kinase p85 Alpha (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug.

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60225-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, fetal human brain tissue, Jurkat cells, MCF-10A cells, mouse brain tissue, Neuro-2a cells, PC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, U-251 cells, HT-29 cells, HuT 78 cells, C2C12 cells
• Positive IP detected in: RAW 264.7 cells
• Positive IHC detected in: mouse lung tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 178 publications below
• IHC: See 9 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

60225-1-Ig targets PI3 Kinase p85 Alpha in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat, pig, canine, bovine, duck
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p85 Alpha fusion protein Ag2344
• Full Name: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC030815
• Gene Symbol: PI3 Kinase p85 Alpha
• Gene ID (NCBI): 5295
• RRID: AB_11042594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

Publications

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human	WB	Autophagy; The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis.; Authors - Shulan Pi
human	WB	Oncogene; MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.; Authors - Ran-Ran Ma
mouse	WB	Phytother Res; 11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2 /ERβ signaling pathways; Authors - Yan Lyu
rat	WB	Environ Pollut; Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.; Authors - Rundong Liu
mouse	WB	Arterioscler Thromb Vasc Biol; Short-Term Exposure to Waterpipe/Hookah Smoke Triggers a Hyperactive Platelet Activation State and Increases the Risk of Thrombogenesis.; Authors - Ahmed B Alarabi

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