c-SRC Polyclonal antibody

c-SRC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

66

Cat no : 11097-1-AP

Synonyms

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Validation Data Gallery

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  WB analysis using 11097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11097-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of CHO using 11097-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11097-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11097-1-AP

  IP result of anti-c-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 11097-1-AP

  Immunofluorescent analysis of A431 cells using 11097-1-AP (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 11097-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug c-SRC antibody (11097-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse brain tissue, COLO 320 cells, Jurkat cells, A431 cells, CHO cells, SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human breast cancer tissue, human brain tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 56 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Product Information

11097-1-AP targets c-SRC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

• Tested Reactivity: human, mouse, rat, hamster
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-SRC fusion protein Ag1575
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 55 kDa, 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• RRID: AB_2286705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Publications

Species	Application	Title
human	WB	Nat Genet; Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation.; Authors - Jian Zheng
mouse	WB,IP	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
		J Cell Biol; Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence.; Authors - Karim El Azzouzi
human		Cell Death Differ; The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein.; Authors - Lihui Zhu
mouse	WB	Haematologica; NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis.; Authors - Jianlin Qiao

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