Validation Data Gallery
|Positive WB detected in||Jurkat cells, A431 cells, CHO cells, COLO 320 cells, mouse brain tissue, SH-SY5Y cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||human breast cancer tissue, human brain tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:300-1:800|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11097-1-AP targets SRC in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
|Tested Reactivity||human, mouse, rat, hamster|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SRC fusion protein Ag1575|
|Full Name||v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||55 kDa, 60 kDa|
|GenBank accession number||BC011566|
|Gene ID (NCBI)||6714|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).
|Product Specific Protocols|
|WB protocol for SRC antibody 11097-1-AP||Download protocol|
|IHC protocol for SRC antibody 11097-1-AP||Download protocol|
|IF protocol for SRC antibody 11097-1-AP||Download protocol|
|IP protocol for SRC antibody 11097-1-AP||Download protocol|
|FC protocol for SRC antibody 11097-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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