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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PFKFB3

PFKFB3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 13763-1-AP

Synonymes

6PF 2 K/Fru 2,6 P2ase 3, IPFK2, PFK/FBPase 3, PFK2, PFKFB3, PFKFB3-Specific



Applications testées

Résultats positifs en WBcellules HEK-293, cellules A431, cellules HeLa, cellules Jurkat, tissu cardiaque de souris, tissu de thymus de souris, tissu splénique de souris
Résultats positifs en IPtissu splénique de souris,
Résultats positifs en IHCtissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13763-1-AP cible PFKFB3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PFKFB3 Protéine recombinante Ag4744
Nom complet 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Masse moléculaire calculée 520 aa, 60 kDa
Poids moléculaire observé 58 kDa
Numéro d’acquisition GenBankBC040482
Symbole du gène PFKFB3
Identification du gène (NCBI) 5209
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

Protocole

Product Specific Protocols
WB protocol for PFKFB3 antibody 13763-1-APDownload protocol
IHC protocol for PFKFB3 antibody 13763-1-APDownload protocol
IF protocol for PFKFB3 antibody 13763-1-APDownload protocol
IP protocol for PFKFB3 antibody 13763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

FGF-dependent metabolic control of vascular development.

Authors - Pengchun Yu
humanWB

Cell

Role of PFKFB3-Driven Glycolysis in Vessel Sprouting.

Authors - De Bock Katrien K
humanWB

Cell Metab

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Authors - Qidong Li
humanWB,IP

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseWB

Circulation

Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth.

Authors - Andrew A Gibb
mouseWB

ACS Nano

Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Authors - Mengshi Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-24-2022)

this antibody is working good for westerner blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and colon cancer cell line
FH

Balawant (Verified Customer) (05-08-2022)

tis antibody is working great at 1:2000 diluton. I have used this antibody from total cells lysate of colonic epithelial cells form human origin.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colonic epithelial cells
FH

Iram (Verified Customer) (04-13-2022)

Very good signal

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Colon cancer cells
FH

Susmita (Verified Customer) (03-11-2022)

This antibody works great for me.

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: PC cell line
FH

Hsin-Sheng (Verified Customer) (08-19-2019)

Strong bend intensity at around 60 kD. However, there is a non-specific band near 65-70 kD.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1;200
  • Cell Tissue Type: H1299