SKP1 Polyclonal antibody

SKP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10990-2-AP


EMC19, NET19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

Tested Applications

Positive WB detected inHeLa cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10990-2-AP targets SKP1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKP1 fusion protein Ag1457
Full Name S-phase kinase-associated protein 1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC009839
Gene Symbol SKP1
Gene ID (NCBI) 6500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.


Product Specific Protocols
WB protocol for SKP1 antibody 10990-2-APDownload protocol
IHC protocol for SKP1 antibody 10990-2-APDownload protocol
IF protocol for SKP1 antibody 10990-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.

Authors - Xiao-Tong Lin

Pharmacol Res

Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.

Authors - Shangping Xing

Mol Nutr Food Res

Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer

Authors - Da Huang

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song
humanIHC, WB

J Exp Clin Cancer Res

Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.

Authors - He Zhu


Recruitment of FBXO22 for Targeted Degradation of NSD2

Authors - David Y Nie


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


WEI (Verified Customer) (05-11-2022)

Weak band but at correct size.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart