SKP1 Polyclonal antibody

SKP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10990-2-AP

Synonyms

OCP2, OCP II, OCP 2, NET19, EMC19

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Validation Data Gallery

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  WB analysis using 10990-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10990-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10990-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10990-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SKP1 antibody (10990-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue
• Positive IHC detected in: human gliomas tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

10990-2-AP targets SKP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKP1 fusion protein Ag1457
• Full Name: S-phase kinase-associated protein 1
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC009839
• Gene Symbol: SKP1
• Gene ID (NCBI): 6500
• RRID: AB_2187492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Publications

Species	Application	Title
human	WB	Elife; Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.; Authors - Xiao-Tong Lin
human	WB,IHC	Pharmacol Res; Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.; Authors - Shangping Xing
human	WB	Mol Nutr Food Res; Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer; Authors - Da Huang
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	IHC, WB	J Exp Clin Cancer Res; Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.; Authors - He Zhu
human	WB	bioRxiv; Recruitment of FBXO22 for Targeted Degradation of NSD2; Authors - David Y Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Weak band but at correct size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart