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  • KD/KO Validated

PFKP Polyclonal antibody

PFKP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13389-1-AP


6 phosphofructokinase type C, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected inJurkat cells, mouse heart tissue
Positive IHC detected inmouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, BxPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13389-1-AP targets PFKP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKP fusion protein Ag4047
Full Name phosphofructokinase, platelet
Calculated Molecular Weight 784 aa, 86 kDa
Observed Molecular Weight 80-86 kDa
GenBank Accession NumberBC029138
Gene Symbol PFKP
Gene ID (NCBI) 5214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).


Product Specific Protocols
WB protocol for PFKP antibody 13389-1-APDownload protocol
IHC protocol for PFKP antibody 13389-1-APDownload protocol
IF protocol for PFKP antibody 13389-1-APDownload protocol
IP protocol for PFKP antibody 13389-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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