Hexokinase 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Hexokinase 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

255

Reviews (4)

Cat no : 22029-1-AP

Synonyms

Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase

---

Validation Data Gallery

• 

  WB analysis using 22029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22029-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22029-1-AP (Hexokinase 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22029-1-AP

  IP result of anti-Hexokinase 2 (IP:22029-1-AP, 4ug; Detection:22029-1-AP 1:10000) with HeLa cells lysate 1520 ug.

• 

  IHC staining of human urothelial carcinoma using 22029-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 22029-1-AP (Hexokinase 2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, Jurkat cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 231 publications below
• IHC: See 42 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Product Information

22029-1-AP targets Hexokinase 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine, monkey, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hexokinase 2 fusion protein Ag16895
• Full Name: hexokinase 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC021116
• Gene Symbol: Hexokinase 2
• Gene ID (NCBI): 3099
• RRID: AB_11182717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human,mouse	WB	Cell Metab; An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.; Authors - Nunu Huang
mouse	WB	Cell Metab; STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.; Authors - Chunyan Zhang
	IP	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human,mouse	WB,IHC	Adv Sci (Weinh); Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis; Authors - Bin Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

SONAM (Verified Customer) (02-22-2024)

Works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells, macrophages

FH

Maialen (Verified Customer) (10-03-2023)

It works very well

• Applications: Immunoprecipitation

Hexokinase 2 Antibody Immunoprecipitation validation ( dilution) in (Cat no:22029-1-AP)

FH

Macarena (Verified Customer) (10-07-2022)

excellent results. Very specific

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cell lines

FH

Henar (Verified Customer) (08-31-2022)

Great antibody! No background and very specific result.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary mouse neurons

Related Products

GPI Polyclonal antibody

15171-1-AP

FBP1 Polyclonal antibody

12842-1-AP

PFKP Polyclonal antibody

13389-1-AP

FBP2 Polyclonal antibody

25192-1-AP

PGM1 Polyclonal antibody

15161-1-AP