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  • KD/KO Validated

Hexokinase 2 Polyclonal antibody

Hexokinase 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 22029-1-AP

Synonyms

Hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase



Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, Jurkat cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22029-1-AP targets Hexokinase 2 in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hexokinase 2 fusion protein Ag16895
Full Name hexokinase 2
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol HK2
Gene ID (NCBI) 3099
RRIDAB_11182717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.

Protocols

Product Specific Protocols
WB protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IHC protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
IP protocol for Hexokinase 2 antibody 22029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Metab

An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.

Authors - Nunu Huang
mouseWB

Cell Metab

STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.

Authors - Chunyan Zhang
IP

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
mouseWB

Nat Neurosci

Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.

Authors - Xiaoxiang Dong
mouseWB,IF

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang
humanWB,IF,IHC

Nat Commun

SUMOylation controls the binding of hexokinase 2 to mitochondria and protects against prostate cancer tumorigenesis.

Authors - Xun Shangguan
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SONAM (Verified Customer) (02-22-2024)

Works well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cancer cells, macrophages
FH

Maialen (Verified Customer) (10-03-2023)

It works very well

  • Applications: Immunoprecipitation
Hexokinase 2 Antibody Immunoprecipitation validation ( dilution) in (Cat no:22029-1-AP)
FH

Macarena (Verified Customer) (10-07-2022)

excellent results. Very specific

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cell lines
FH

Henar (Verified Customer) (08-31-2022)

Great antibody! No background and very specific result.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary mouse neurons