Anticorps Polyclonal de lapin anti-Integrin Beta 1

• Phare
• Validé par KD/KO

Integrin Beta 1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 26918-1-AP

Synonymes

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

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Galerie de données de validation

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  WB analysis using 26918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin Beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 26918-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26918-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26918-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HT-1080, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu cutané humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Informations sur le produit

26918-1-AP cible Integrin Beta 1 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Beta 1 Protéine recombinante Ag25426
• Nom complet: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC020057
• Symbole du gène: ITGB1
• Identification du gène (NCBI): 3688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
rat	WB	Theranostics; A functionalized self-assembling peptide containing E7 and YIGSR sequences enhances neuronal differentiation of spermatogonial stem cells on aligned PCL fibers for spinal cord injury repair; Authors - Zhiyuan Wang
human	WB,IF	Mater Today Bio; Carbon dots enhance extracellular matrix secretion for dentin-pulp complex regeneration through PI3K/Akt/mTOR pathway-mediated activation of autophagy.; Authors - Lili Liu
rat	WB	ACS Appl Mater Interfaces; Engineered Biomimetic Nanoplatform Protects the Myocardium Against Ischemia/Reperfusion Injury by Inhibiting Pyroptosis.; Authors - Yazhong Wei
human	WB	Cell Chem Biol; Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.; Authors - David W Wolff
mouse	WB,IHC	Biomed Pharmacother; Triptolide attenuates pulmonary fibrosis by inhibiting fibrotic extracellular matrix remodeling mediated by MMPs/LOX/integrin; Authors - Weiji Lin

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