Integrin alpha-5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin alpha-5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (1)

Cat no : 10569-1-AP

Synonyms

ITGA5, Integrin alpha-F, Integrin alpha-5 light chain, Integrin alpha-5 heavy chain, FNRA

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Validation Data Gallery

• 

  WB analysis of HeLa using 10569-1-AP

  WB result of Integrin alpha-5 antibody (10569-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

• 

  WB analysis using 10569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10569-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10569-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 10569-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 10569-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Product Information

10569-1-AP targets Integrin alpha-5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin alpha-5 fusion protein Ag0860
• Full Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 135-150 kDa
• GenBank Accession Number: BC008786
• Gene Symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• RRID: AB_2130060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Publications

Species	Application	Title
		Biomaterials; Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.; Authors - Yonglin Yu
mouse	WB	Int J Mol Sci; Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway; Authors - Zanyan Zhao
human	WB,IF	Front Cell Dev Biol; Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.; Authors - Chengyun Pan
human	WB	Oncotarget; Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.; Authors - Huimin Wang
		Sci Rep; Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.; Authors - Mayra Rachele Zarone
human	IF	Front Oncol; ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas.; Authors - Shuyu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (02-04-2019)

Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.

• Applications: Western Blot, Immunofluorescence,

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