CD41/Integrin alpha 2b Polyclonal antibody

CD41/Integrin alpha 2b Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 24552-1-AP

Synonyms

CD41, CD41/Integrin Alpha 2B, CD41B, GP2B, GPalpha IIb, GPIIb, GTA, HPA3, Integrin alpha IIb, ITGA2B, ITGAB

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Validation Data Gallery

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  WB analysis using 24552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat blood using 24552-1-AP

  mouse spleen and rat blood were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin alpha 2b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 24552-1-AP

  IP result of anti-CD41/Integrin Alpha 2B (IP:24552-1-AP, 4ug; Detection:24552-1-AP 1:2000) with human plasma lysate 4000ug.

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  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

• 

  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse lung tissue, rat blood, mouse spleen tissue, rat spleen tissue, human blood tissue
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: mouse spleen tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Product Information

24552-1-AP targets CD41/Integrin alpha 2b in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD41/Integrin alpha 2b fusion protein Ag19459
• Full Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Calculated Molecular Weight: 1039 aa, 113 kDa
• Observed Molecular Weight: 110-125 kDa
• GenBank Accession Number: BC126442
• Gene Symbol: CD41a
• Gene ID (NCBI): 3674
• RRID: AB_2879604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.

Publications

Species	Application	Title
human	WB	Sci Adv; Platelet-derived porous nanomotor for thrombus therapy.; Authors - Mimi Wan
human,mouse	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
mouse	WB	Theranostics; Platelets camouflaged nanovehicle improved bladder cancer immunotherapy by triggering pyroptosis; Authors - Jiale Tian
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen
human	WB	Mol Ther; Disruption of Chromosomal Architecture of cox2 Locus Sensitizes Lung Cancer Cells to Radiotherapy.; Authors - Yuxiang Sun

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