Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19676-1-AP targets Integrin alpha 4 in WB, IHC, IF, ELISA, Blocking assay applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | NM_000885 |
| Gene Symbol | Integrin alpha 4 |
| Gene ID (NCBI) | 3676 |
| RRID | AB_10640907 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrin alpha 4 (ITGA4), also named CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1, and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin alpha 4 antibody 19676-1-AP | Download protocol |
| WB protocol for Integrin alpha 4 antibody 19676-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-VCAM-1 antibody (Cat# 19676-1-AP) has accumulated 33 citations across journals including Nature Communications, Advanced Science, Journal of Nanobiotechnology, Cancer Letters, Journal of Experimental Medicine, and International Journal of Molecular Sciences. Research fields span nanomedicine and biomimetic drug delivery, oncology (breast, glioma, melanoma, bladder, gastric, and thyroid cancers), inflammation (IBD, atherosclerosis, pulmonary), neurology (stroke), liver fibrosis, cardiovascular disease, and cell adhesion biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. | ||
J Nanobiotechnology Macrophage-cancer hybrid membrane-coated nanoparticles for targeting lung metastasis in breast cancer therapy. | ||
J Nanobiotechnology Biomimetic liposomal nanozymes improve breast cancer chemotherapy with enhanced penetration and alleviated hypoxia | ||
J Nanobiotechnology Cell membrane-coated human hair nanoparticles for precise disease therapies | ||
J Exp Clin Cancer Res Tumor-intrinsic ENO1 inhibition promotes antitumor immune response and facilitates the efficacy of anti-PD-L1 immunotherapy in bladder cancer | ||
Adv Sci (Weinh) A pH-Responsive Biomimetic Antioxidant Nanoplatform with Dual Renal Targeting for Synergistic Therapy of Acute Kidney Injury. |
Reviews
What customers say
One reviewer (Boyan Zhang, 2019) rated this product 3/5 stars. For Western Blot on MEF cells, the expected band was very weak and much fainter than non-specific bands. For Immunofluorescence, the antibody successfully labeled the cell cortex localization of Itga4 and partially co-localized with Actin. Overall, performance was mixed: acceptable for IF but suboptimal for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (03-11-2019) | For WB, the expected band was very weak, and was much weaker than those non-specific bands; for IF, it could label the cell cortex localisation of Itga4, partially co-localised with Actin.
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