Integrin Alpha 3 Polyclonal antibody

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• KD/KO Validated

Integrin Alpha 3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21992-1-AP

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Validation Data Gallery

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  WB analysis of A549 using 21992-1-AP

  WB result of Integrin Alpha 3 antibody (21992-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

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  WB analysis using 21992-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21992-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 21992-1-AP

  IP result of anti-Integrin Alpha 3 (IP:21992-1-AP, 4ug; Detection:21992-1-AP 1:500) with PC-3 cells lysate 1200ug.

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 21992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Integrin Alpha 3 antibody (21992-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, human heart tissue, human testis tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

21992-1-AP targets Integrin Alpha 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Calculated Molecular Weight: 1066 aa, 119 kDa
• Observed Molecular Weight: 130-160 kDa
• GenBank Accession Number: BC150190
• Gene Symbol: Integrin alpha 3
• Gene ID (NCBI): 3675
• RRID: AB_10953527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publications

Species	Application	Title
human	WB	Front Oncol; High Expression of Integrin α3 Predicts Poor Prognosis and Promotes Tumor Metastasis and Angiogenesis by Activating the c-Src/Extracellular Signal-Regulated Protein Kinase/Focal Adhesion Kinase Signaling Pathway in Cervical Cancer.; Authors - Qiqiao Du
human,mouse	WB,IF	Nat Commun; Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.; Authors - Eunnyung Bae
human	IF	Front Microbiol; Engagement of α3β1 and α2β1 integrins by hypervirulent Streptococcus agalactiae in invasion of polarized enterocytes; Authors - Giuseppe Valerio De Gaetano
human		Small; Mimicking Tumor Metastasis Using a Transwell-Integrated Organoids-On-a-Chip Platform; Authors - Maike Chen

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