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Integrin Beta 1 Monoclonal antibody

Integrin Beta 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A7C9

Cat no : 66315-1-Ig

Synonyms

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HUVEC using 66315-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHT-1080 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66315-1-Ig targets Integrin Beta 1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin Beta 1 fusion protein Ag24476
Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC020057
Gene Symbol Integrin beta 1
Gene ID (NCBI) 3688
RRIDAB_2881696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 1 antibody 66315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Structure

Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.

Authors - Eun-Ah Cho
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina/Brain
Integrin Beta 1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)