Integrin Alpha 3 Monoclonal antibody

• Featured Product
• KD/KO Validated

Integrin Alpha 3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

CloneNo.

3F9G4

Reviews (3)

Cat no : 66070-1-Ig

Synonyms

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Validation Data Gallery

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  WB analysis of A549 using 66070-1-Ig

  WB result of Integrin Alpha 3 antibody (66070-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

• 

  WB analysis of PC-3 using 66070-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (ITGA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66070-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66070-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66070-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66070-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66070-1-Ig (Integrin alpha-3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• FC: See 2 publications below

Product Information

66070-1-Ig targets Integrin Alpha 3 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Cited Reactivity: human, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Calculated Molecular Weight: 1066 aa, 119 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC150190
• Gene Symbol: Integrin alpha 3
• Gene ID (NCBI): 3675
• RRID: AB_11042765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publications

Species	Application	Title
human	WB, IF	Mol Cell Proteomics; Hypoxia-induced changes to integrin alpha 3 glycosylation facilitate invasion in epidermoid carcinoma cell line A431.; Authors - Yan Ren
mouse	WB	CNS Neurosci Ther; Integrin α3 is required for high-frequency repetitive transcranial magnetic stimulation-induced glutamatergic synaptic transmission in mice with ischemia; Authors - Li Liu
human	WB	Am J Physiol Cell Physiol; ITGA3 participates in the pathogenesis of recurrent spontaneous abortion by downregulating ULK1-mediated autophagy to inhibiting trophoblast function; Authors - Rui Qi Wang
human	IHC	Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo
human	IHC	Front Mol Biosci; Golgi Phosphoprotein 3 Promotes Colon Cancer Cell Metastasis Through STAT3 and Integrin α3 Pathways.; Authors - Anpei Huang
human	FC	Cell Adh Migr; Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions.; Authors - Yanling Jin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ewan (Verified Customer) (07-11-2024)

Works well by western clean band and correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 O/N @ 4 degrees
• Cell Tissue Type: MDA-MB-231

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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