Anticorps Polyclonal de lapin anti-IL-18

• Phare
• Validé par KD/KO

IL-18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

250

N° de cat : 10663-1-AP

Synonymes

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

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Galerie de données de validation

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10663-1-AP

  WB result of Pre-IL18 antibody (10663-1-AP, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells..

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10663-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10663-1-AP

  IP result of anti-IL-18 (IP:10663-1-AP, 4ug; Detection:10663-1-AP 1:5000) with HeLa cells lysate 1360 ug.

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  IHC staining of human tonsillitis using 10663-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10663-1-AP (IL18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10663-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10663-1-AP (IL18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (10663-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of HeLa cells using 10663-1-AP (Pre-IL18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10663-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human IL-18 (10663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules DU 145, cellules HaCaT, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 227 publications below
• IHC: See 42 publications below
• IF: See 22 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10663-1-AP cible IL-18 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-18 Protéine recombinante Ag1063
• Nom complet: IL-18
• Masse moléculaire calculée: 193 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC007461
• Symbole du gène: IL-18
• Identification du gène (NCBI): 3606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Publications

Species	Application	Title
mouse	IF	Sci Adv; FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity.; Authors - Jun Ren
mouse	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
mouse	WB	Nat Commun; IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.; Authors - Hung-Yu Chiang
human,mouse	WB	J Pineal Res; Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation; Authors - Zitong Wang
mouse	WB，IHC	Aging Dis; Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.; Authors - Danyang Li
human	WB	Proc Natl Acad Sci U S A; Telomere dysfunction instigates inflammation in inflammatory bowel disease.; Authors - Deepavali Chakravarti

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