IL-18 Polyclonal antibody

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10663-1-AP

  WB result of IL-18 antibody (10663-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells.

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10663-1-AP

  IP result of anti-IL-18 (IP:10663-1-AP, 4ug; Detection:10663-1-AP 1:5000) with HeLa cells lysate 1360ug.

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  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (10663-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of HeLa cells using 10663-1-AP (Pre-IL18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10663-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human IL-18 (10663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

The IL-18 polyclonal antibody (10663-1-AP) is the highest cited IL-18 antibody in the market with 378 citations.

Cat No. 10663-1-AP

Publications(378)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

IL18, Pre-IL18, Iboctadekin, IFN-gamma-inducing factor, IL 1 gamma

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, DU 145 cells, HaCaT cell, HaCaT cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 343 publications below
• IHC: See 53 publications below
• IF: See 35 publications below
• IP: See 1 publications below

Product Information

10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1063

  Product name: Recombinant human Pre IL-18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of BC007461

  Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL-18
• Gene ID (NCBI): 3606
• RRID: AB_2123636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Sci Adv; FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity.; Authors - Jun Ren
	WB	Nat Commun; IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.; Authors - Hung-Yu Chiang
	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
human	WB	J Pineal Res; Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation; Authors - Zitong Wang
	WB,IHC	Aging Dis; Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.; Authors - Danyang Li
human	WB	Proc Natl Acad Sci U S A; Telomere dysfunction instigates inflammation in inflammatory bowel disease.; Authors - Deepavali Chakravarti

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jillian (Verified Customer) (05-27-2025)

Great antibody, labels very well. We had to titrate multiple times to get to the working value because it had a high background until we reached 1:5000 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Frontal, ICJ