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IL-18 Polyclonal antibody

IL-18 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10663-1-AP

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma



Tested Applications

Positive WB detected inA431 cells, HeLa cells, NIH/3T3 cells, DU 145 cells, HaCaT cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-18 fusion protein Ag1063
Full Name IL-18
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007461
Gene Symbol IL-18
Gene ID (NCBI) 3606
RRIDAB_2123636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Protocols

Product Specific Protocols
WB protocol for IL-18 antibody 10663-1-APDownload protocol
IHC protocol for IL-18 antibody 10663-1-APDownload protocol
IF protocol for IL-18 antibody 10663-1-APDownload protocol
IP protocol for IL-18 antibody 10663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity.

Authors - Jun Ren
mouseWB

Nat Commun

Telomere dysfunction activates YAP1 to drive tissue inflammation.

Authors - Deepavali Chakravarti
mouseWB

Nat Commun

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Authors - Hung-Yu Chiang
human,mouseWB

J Pineal Res

Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation

Authors - Zitong Wang
mouseWB,IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li
humanWB

Proc Natl Acad Sci U S A

Telomere dysfunction instigates inflammation in inflammatory bowel disease.

Authors - Deepavali Chakravarti