IL-18 Polyclonal antibody

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• IL-18 Polyclonal antibody (10663-1-AP)

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• KD/KO Validated

The IL-18 polyclonal antibody (10663-1-AP) is the highest cited IL-18 antibody in the market with 518 citations.

Cat No. 10663-1-AP

Publications(518)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

IL18, Pre-IL18, Iboctadekin, IFN-gamma-inducing factor, IL 1 gamma

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  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10663-1-AP

  WB result of IL-18 antibody (10663-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL-18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10663-1-AP (IL18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10663-1-AP

  IP result of anti-IL-18 (IP:10663-1-AP, 4ug; Detection:10663-1-AP 1:5000) with HeLa cells lysate 1360ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (10663-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10663-1-AP

  Immunofluorescent analysis of HeLa cells using 10663-1-AP (Pre-IL18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 10663-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human IL-18 (10663-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, DU 145 cells, HaCaT cell, HaCaT cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, chicken, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1063

  Product name: Recombinant human Pre IL-18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of BC007461

  Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL-18
• Gene ID (NCBI): 3606
• RRID: AB_2123636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell; Targeting peripheral 5-HT(2A)R enhances antitumor immunity in colorectal cancer.; Authors - YaTing Wen
	WB	Mol Cancer; Anti irradiation nanoparticles shelter immune organ from radio-damage via preventing the IKK/IκB/NF-κB activation; Authors - Shigao Huang
	WB	Cell Mol Immunol; Pik3c3 deficiency in myeloid cells imparts partial resistance to experimental autoimmune encephalomyelitis associated with reduced IL-1β production.; Authors - Guan Yang ; KD Validated
	WB	Nat Commun; Telomere dysfunction activates YAP1 to drive tissue inflammation.; Authors - Deepavali Chakravarti
	IF	Nat Commun; Mechanics-activated fibroblasts promote pulmonary group 2 innate lymphoid cell plasticity propelling silicosis progression; Authors - Yangyang He
	WB	Nat Commun; Loss of DNA methyltransferase 3a enhances Caspase-8 transcription and promotes cardiomyocyte pyroptosis during myocardial infarction.; Authors - Ning Wang

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Jillian (Verified Customer) (05-27-2025)

Great antibody, labels very well. We had to titrate multiple times to get to the working value because it had a high background until we reached 1:5000 dilution

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Frontal, ICJ