IL-12/IL-23 p40 Monoclonal antibody
IL-12/IL-23 p40 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
4B2A8
Cat no : 66288-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human liver tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66288-1-Ig targets IL-12/IL-23 p40 in WB, ELISA applications and shows reactivity with human, pig samples.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-12/IL-23 p40 fusion protein Ag21429 |
Full Name | interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) |
Calculated Molecular Weight | 328 aa, 37 kDa |
Observed Molecular Weight | 37 kDa |
GenBank Accession Number | BC067498 |
Gene Symbol | IL12B |
Gene ID (NCBI) | 3593 |
RRID | AB_2881671 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).
Protocols
Product Specific Protocols | |
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WB protocol for IL-12/IL-23 p40 antibody 66288-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |