IL-12A Monoclonal antibody

IL-12A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A10A8

Cat no : 60324-1-Ig

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12A, IL-12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60324-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60324-1-Ig (IL12A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60324-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IHC detected in: human kidney tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60324-1-Ig targets IL-12A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-12A fusion protein Ag17625
• Full Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC104982
• Gene Symbol: IL-12A
• Gene ID (NCBI): 3592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.