IL-10 Polyclonal antibody

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  WB analysis of Recombinant protein using 20850-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 20850-1-AP (IL-10 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IL-10 Polyclonal Antibody for WB, ELISA

Cat No. 20850-1-AP

Publications(117)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, ELISA

Synonyms

IL-10,IL10, interleukin 10, IL10A, IL10, IL 10

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Tested Applications

• Positive WB detected in: Recombinant protein, ag14644

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 78 publications below
• IHC: See 26 publications below
• IF: See 14 publications below
• ELISA: See 1 publications below

Product Information

20850-1-AP targets IL-10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-10 fusion protein Ag14644
• Full Name: interleukin 10
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC104252
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• RRID: AB_2878752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22301
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Publications

Species	Application	Title
mouse	IF	Adv Sci (Weinh); 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure; Authors - Jiabin Zhang
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
mouse	IHC	Microbiome; Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.; Authors - Hong Zheng
rat	IHC	Acta Biomater; A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.; Authors - Yingzheng Zhao
rat	IHC	Acta Biomater; Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization; Authors - Dan Qiao
mouse	WB	Alzheimers Res Ther; Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis; Authors - Deng-Pan Wu