Anticorps Monoclonal anti-IFITM1-Specific

• Phare
• Validé par KD/KO

IFITM1-Specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

66

CloneNo.

5B5E2

Avis (1)

N° de cat : 60074-1-Ig

Synonymes

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13

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Galerie de données de validation

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  WB analysis of TF-1 using 60074-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60074-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60074-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60074-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60074-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60074-1-Ig (IFITM1-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 60074-1-Ig

  IP Result of anti-IFITM1-Specific (IP:60074-1-Ig, 4ug; Detection:60074-1-Ig 1:500) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human lymphoma using 60074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60074-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60074-1-Ig (IFITM1-Specific Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of K-562 using 60074-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IFITM1-Specific antibody (60074-1-Ig, Clone: 5B5E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of U2OS using 60074-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human IFITM1-Specific (60074-1-Ig, Clone:5B5E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules TF-1, cellules HepG2, cellules HL-60, cellules K-562, tissu testiculaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules K-562,
• Résultats positifs en cytométrie: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 59 publications below
• IHC: See 3 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Informations sur le produit

60074-1-Ig cible IFITM1-Specific dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IFITM1-Specific Protéine recombinante Ag2320
• Nom complet: interferon induced transmembrane protein 1 (9-27)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC000897
• Symbole du gène: IFITM1
• Identification du gène (NCBI): 8519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

Publications

Species	Application	Title
human	WB,FC	Science; IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.; Authors - Julian Buchrieser
human	WB	Cell; The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.; Authors - Abraham L Brass
human	WB	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo
human	WB	Sci Transl Med; Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.; Authors - Mohammed Selman
human	WB	Cell Host Microbe; Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.; Authors - Toshana L Foster ; KD Validated
human	IF	Gastroenterology; PMID: 23123437; Authors - Daryl T-Y Lau

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Claudia (Verified Customer) (06-20-2022)

The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:400 (immunofluorescence), 1:20.000 (Western blot)
• Cell Tissue Type: Permanent cell lines

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