Anticorps Polyclonal de lapin anti-IFIT2

• Phare
• Validé par KD/KO

IFIT2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

42

N° de cat : 12604-1-AP

Synonymes

cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54

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Galerie de données de validation

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  WB analysis of A431 cells using 12604-1-AP

  WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.

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  WB analysis using 12604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 12604-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12604-1-AP

  IP result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:2000) with A431 cells lysate 1200 ug.

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12604-1-AP

  Immunofluorescent analysis of HepG2 cells using 12604-1-AP (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules RAW 264.7, cellules THP-1
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu de cancer de la peau humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 40 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

12604-1-AP cible IFIT2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFIT2 Protéine recombinante Ag3283
• Nom complet: IFIT 2
• Masse moléculaire calculée: 484 aa, 56 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC032839
• Symbole du gène: IFIT2
• Identification du gène (NCBI): 3433
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Mol Cell; Translation stress and collided ribosomes are co-activators of cGAS.; Authors - Li Wan
human	WB	Nat Microbiol; ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.; Authors - GuanQun Liu
human	WB	Nat Microbiol; Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.; Authors - Vy Tran
		Nucleic Acids Res; IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.; Authors - Renata C Fleith

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