IRF7 Polyclonal antibody

IRF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 22392-1-AP

Synonyms

IRF7A, IRF-7, IRF 7H, IRF 7

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Validation Data Gallery

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  WB analysis using 22392-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22392-1-AP (IRF7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 22392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22392-1-AP (IRF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 22392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22392-1-AP (IRF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

22392-1-AP targets IRF7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, paralichthys olivaceus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF7 fusion protein Ag18059
• Full Name: IRF 7
• Calculated Molecular Weight: 516 aa, 56 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC136555
• Gene Symbol: IRF7
• Gene ID (NCBI): 3665
• RRID: AB_2879097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).This antibody is a rabbit polyclonal antibody raised against the C-terminal 349 amino acid residues of human IRF7 D. The molecular weight of Nonphosphorylated IRF7 cofractionated with the 44-kDa marker, approximating its predicted size. In contrast, phosphorylated IRF7, which migrate more slowly on SDS-PAGE. This phosphorylated IRF7 was consistent with a size of 80 to 90 kDa. (PMID: 11073981 )

Publications

Species	Application	Title
human	WB	Cancer Lett; NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway; Authors - Jinghan Zhu
human	WB	Cell Chem Biol; Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses.; Authors - Ghizlane Maarifi
human	WB	J Immunol; Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.; Authors - Zhangchuan Xia
human	WB	J Immunol; Inducible ATP1B1 Upregulates Antiviral Innate Immune Responses by the Ubiquitination of TRAF3 and TRAF6.; Authors - Wei Cao
mouse	WB	Phytomedicine; Si-Ni-San ameliorates chronic colitis by modulating type I interferons-mediated inflammation.; Authors - Yajie Cai
human	WB	Cancer Med; A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response.; Authors - Xiao-Ming Lyu