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IRF9 Polyclonal antibody

IRF9 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14167-1-AP


IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48

Tested Applications

Positive WB detected inA549 cells, mouse heart tissue, rat heart tissue, HepG2 cells, MCF-7 cells, HeLa cells, THP-1 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14167-1-AP targets IRF9 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF9 fusion protein Ag5365
Full Name IRF 9
Calculated Molecular Weight 393 aa, 44 kDa
Observed Molecular Weight 44-48 kDa
GenBank Accession NumberBC035716
Gene Symbol IRF9
Gene ID (NCBI) 10379
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.


Product Specific Protocols
WB protocol for IRF9 antibody 14167-1-APDownload protocol
IHC protocol for IRF9 antibody 14167-1-APDownload protocol
IF protocol for IRF9 antibody 14167-1-APDownload protocol
IP protocol for IRF9 antibody 14167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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