IRF9 Polyclonal antibody

• Featured Product
• KD/KO Validated

IRF9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 14167-1-AP

Synonyms

ISGF3G, ISGF-3 gamma, IRF-9, IRF 9, Interferon-stimulated gene factor 3 gamma

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Validation Data Gallery

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14167-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 14167-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14167-1-AP

  IP result of anti-IRF9 (IP:14167-1-AP, 4ug; Detection:14167-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14167-1-AP

  Immunofluorescent analysis of HepG2 cells, using IRF9 antibody 14167-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: A549 cells, mouse heart tissue, rat heart tissue, HepG2 cells, MCF-7 cells, HeLa cells, THP-1 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Product Information

14167-1-AP targets IRF9 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF9 fusion protein Ag5365
• Full Name: IRF 9
• Calculated Molecular Weight: 393 aa, 44 kDa
• Observed Molecular Weight: 44-48 kDa
• GenBank Accession Number: BC035716
• Gene Symbol: IRF9
• Gene ID (NCBI): 10379
• RRID: AB_2296227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	Cell Rep; miR-342-5p decreases ankyrin G levels in Alzheimer's disease transgenic mouse models.; Authors - Xiaqin Sun
	ChIP	PLoS Pathog; A host cell long noncoding RNA NR_033736 regulates type I interferon-mediated gene transcription and modulates intestinal epithelial anti-Cryptosporidium defense.; Authors - Juan Li
		Front Immunol; PMID: 30158934; Authors - Ming-Hsun Tsai
human	WB	Mol Cancer Ther; JAK2 inhibitor SAR302503 abrogates PD-L1 expression and targets therapy resistant non-small cell lung cancers.; Authors - Sean Pitroda
human	WB, IP, IF	J Immunol; Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.; Authors - Mudan Zhang