Anticorps Polyclonal de lapin anti-MIG6; ERRFI1

• Phare
• Validé par KD/KO

MIG6; ERRFI1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 11630-1-AP

Synonymes

ERRFI1, GENE 33, MIG 6, MIG6, MIG6; ERRFI1, RALT

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Galerie de données de validation

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  WB analysis using 11630-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11630-1-AP (MIG6; ERRFI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11630-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MIG6; ERRFI1 antibody (11630-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11630-1-AP cible MIG6; ERRFI1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIG6; ERRFI1 Protéine recombinante Ag2202
• Nom complet: ERBB receptor feedback inhibitor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC025337
• Symbole du gène: MIG6
• Identification du gène (NCBI): 54206
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

Publications

Species	Application	Title
human	WB	Nat Biomed Eng; Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors.; Authors - Hong-Won Lee
mouse	IHC	Proc Natl Acad Sci U S A; Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation.; Authors - Ben Staal
human	WB	Nucleic Acids Res; A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells.; Authors - Masahiko Ajiro
human	WB	Aging Cell; METTL14 affects UVB-induced human dermal fibroblasts photoaging via miR-100-3p biogenesis in an m6 A-dependent manner; Authors - Lihao Chen
human	IHC	EMBO Rep; MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer.; Authors - Jiabei He
human	WB	Arch Pharm Res; Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC; Authors - Xiaoping Song ; KD Validated

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