AXL Polyclonal antibody

• Featured Product
• KD/KO Validated

AXL Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 13196-1-AP

Synonyms

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

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Validation Data Gallery

• 

  WB analysis of HepG2 using 13196-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13196-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13196-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP (AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13196-1-AP

  IP result of anti-AXL (IP:13196-1-AP, 4ug; Detection:13196-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, HepG2 cells, human liver tissue, HT-1080 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 23 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Product Information

13196-1-AP targets AXL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AXL fusion protein Ag3898
• Full Name: AXL receptor tyrosine kinase
• Calculated Molecular Weight: 894 aa, 98 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC032229
• Gene Symbol: AXL
• Gene ID (NCBI): 558
• RRID: AB_10642006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Publications

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
rat	WB	Int J Mol Sci; Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.; Authors - Mirjana Jerkic
human	WB	Int J Mol Sci; Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.; Authors - Elisa De Tomi
	FC	Bioorg Chem; Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.; Authors - Zhai-Wen Yao
human	WB, IHC,IF	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
human	WB	iScience; SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.; Authors - Kun Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marta (Verified Customer) (05-30-2022)

The antibody works well. Non-specific bands are not detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A375 melanoma cell line

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