EGFR-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

EGFR-Specific Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

135

Reviews (5)

Cat no : 18986-1-AP

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Validation Data Gallery

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  WB analysis using 18986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18986-1-AP

  WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.

• 

  WB analysis of multi-cells using 18986-1-AP

  WB result of anti-EGFR (18986-1-AP) in different cell lysates.

• 

  WB analysis of HeLa/RPE1 cells using 18986-1-AP

  WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.

• 

  WB analysis using 18986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18986-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 18986-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 18986-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 18986-1-AP

  IP Result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

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  IF Staining of A431 using 18986-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EGFR-Specific antibody (18986-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 18986-1-AP

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human EGFR-Specific (18986-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HepG2 cells, multi-cells, HeLa/RPE1 cells, MCF-7 cells, HeLa cells, A431 cells, NCI-H1299 cells, L02 cells, NCL-H1299 cells
• Positive IP detected in: MCF-7 cells
• Positive IF detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:750-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 116 publications below
• IHC: See 19 publications below
• IF: See 16 publications below
• IP: See 8 publications below
• FC: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Product Information

18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, sheep, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated molecular weight: 134 kDa
• Observed molecular weight: 145-165 kDa
• GenBank accession number: NM_005228
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Publications

Species	Application	Title
mouse	WB,IHC	Cell Res; In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.; Authors - Zheng Fu ; KD Validated
human	WB	Cancer Commun (Lond); Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma; Authors - Xiaoteng Cui
human	IF	ACS Appl Mater Interfaces; On-Chip Replication of Extremely Early-Stage Tumor Behavior.; Authors - Chengpan Li
pig	WB	Biosens Bioelectron; Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.; Authors - Yi-Jyun Chen
human	WB, IHC, ChIP	Cancer Res; Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.; Authors - Mahelet Mamo
	WB, IF	Curr Biol; Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.; Authors - Nazim Ali

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (02-13-2023)

A stronger band can be observed slighly above 170 kDa and a fainter band below 130 kDa. Ponceau red is used as total protein loading control. Primary antibody dilution 1:500, overnight incubation at 4ºC. Exposure time 3 minutes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human triple negative breast cancer cells.

EGFR-Specific Antibody Western Blot validation (1:500 dilution) in Human triple negative breast cancer cells. (Cat no:18986-1-AP)

FH

Clarisse (Verified Customer) (12-12-2022)

This antibody works very well on tissue fixed overnight at 4 degrees C with 2% PFA. No antigen retrieval required.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cerebral organoids

FH

Morgan (Verified Customer) (10-26-2021)

I had luck with this antibody as seen from the image attached. It took a little bit of time to develop though. Maybe using more than 1:500 would be better. Unfortunately I couldn't get the phosphorylated version (p-EGF) to work though. I used OV90 (ovarian cancer) cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 ovarian cancer cells

EGFR-Specific Antibody Western Blot validation (1:500 dilution) in OV90 ovarian cancer cells (Cat no:18986-1-AP)

FH

K (Verified Customer) (12-20-2020)

worked well for WB (1:1000)IF (1:200)and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: mouse liver tissues. rat liver and human liver cells

EGFR-Specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation ( dilution) in mouse liver tissues. rat liver and human liver cells (Cat no:18986-1-AP)

FH

XB (Verified Customer) (10-29-2020)

It is OK to use it to probe EGFR (about 180 kD), but there are some unspecific bands at the lower part of the gel.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC12 cell

EGFR-Specific Antibody Western Blot validation (1:1000 dilution) in PC12 cell (Cat no:18986-1-AP)

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