EGFR-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

EGFR-Specific Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

106

Reviews (3)

Cat no : 18986-1-AP

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

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Validation Data Gallery

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  WB analysis using 18986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 18986-1-AP

  WB result of anti-EGFR (18986-1-AP) in different cell lysates.

• 

  WB analysis of HepG2 using 18986-1-AP

  WB result of EGFR-Specific antibody (18986-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EGFR-Specific transfected HepG2 cells.

• 

  WB analysis of HeLa/RPE1 cells using 18986-1-AP

  WB result ofanti-EGFR (18986-1-AP) in Hela and REP1 cell by Dr.Kodani, Andrew.

• 

  WB analysis of MCF-7 using 18986-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 18986-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18986-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18986-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18986-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IP experiment of MCF-7 using 18986-1-AP

  IP Result of anti-EGFR-Specific (IP:18986-1-AP, 3ug; Detection:18986-1-AP 1:500) with MCF-7 cells lysate 2500ug.

• 

  IHC staining of human cervical cancer using 18986-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18986-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18986-1-AP (EGFR-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 18986-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EGFR-Specific antibody (18986-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 18986-1-AP

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human EGFR-Specific (18986-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, NCI-H1299 cells, A431 cells, HeLa cells, L02 cells, HeLa/RPE1 cells, multi-cells, NCL-H1299 cells
• Positive IP detected in: MCF-7 cells
• Positive IF detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:750-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 93 publications below
• IHC: See 14 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated molecular weight: 134 kDa
• Observed molecular weight: 145-165 kDa
• GenBank accession number: NM_005228
• Gene symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERBB1, also mamed as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.; Authors - Zhaoyu Qin
mouse	WB	Ecotoxicol Environ Saf; PMID: 30974244; Authors - Qi Wang
human	WB	Int J Oncol; Adaptive EGF expression sensitizes pancreatic cancer cells to ionizing radiation through activation of the cyclin D1/P53/PARP pathway.; Authors - Xiaoxing Liu
human	IF	Development; The exocyst functions in niche cells to promote germline stem cell differentiation by directly controlling EGFR membrane trafficking.; Authors - Ying Mao
human	WB	Int J Cancer; VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer.; Authors - Yiyu Chen
human	WB	Front Oncol; CAPE-pNO2 Inhibited the Growth and Metastasis of Triple-Negative Breast Cancer via the EGFR/STAT3/Akt/E-Cadherin Signaling Pathway.; Authors - Qin Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Morgan (Verified Customer) (10-26-2021)

I had luck with this antibody as seen from the image attached. It took a little bit of time to develop though. Maybe using more than 1:500 would be better. Unfortunately I couldn't get the phosphorylated version (p-EGF) to work though. I used OV90 (ovarian cancer) cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: OV90 ovarian cancer cells

EGFR-Specific Antibody Western Blot validation (1:500 dilution) in OV90 ovarian cancer cells (Cat no:18986-1-AP)

FH

K (Verified Customer) (12-20-2020)

worked well for WB (1:1000)IF (1:200)and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: mouse liver tissues. rat liver and human liver cells

EGFR-Specific Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation ( dilution) in mouse liver tissues. rat liver and human liver cells (Cat no:18986-1-AP)

FH

XB (Verified Customer) (10-29-2020)

It is OK to use it to probe EGFR (about 180 kD), but there are some unspecific bands at the lower part of the gel.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC12 cell

EGFR-Specific Antibody Western Blot validation (1:1000 dilution) in PC12 cell (Cat no:18986-1-AP)

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