Anticorps Polyclonal de lapin anti-DOCK4

DOCK4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 21861-1-AP

Synonymes

dedicator of cytokinesis 4, DOCK4, KIAA0716

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Galerie de données de validation

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  WB analysis of HEK-293 using 21861-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21861-1-AP (DOCK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21861-1-AP

  IP result of anti-DOCK4 (IP:21861-1-AP, 5ug; Detection:21861-1-AP 1:500) with HeLa cells lysate 1800ug.

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  IP experiment of HEK-293T using 21861-1-AP

  IP result of anti-DOCK4 (IP:21861-1-AP, 4ug; Detection:21861-1-AP 1:300) with HEK-293T cells lysate 4000ug.

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  IHC staining of human ovary tumor using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21861-1-AP (DOCK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 21861-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21861-1-AP (DOCK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T
• Résultats positifs en IP: cellules HeLa, cellules HEK-293T
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer de la prostate humain, tissu de cancer du sein humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:150-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21861-1-AP cible DOCK4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DOCK4 Protéine recombinante Ag16516
• Nom complet: dedicator of cytokinesis 4
• Masse moléculaire calculée: 2011 aa, 230 kDa
• Poids moléculaire observé: 225 kDa
• Numéro d’acquisition GenBank: BC117689
• Symbole du gène: DOCK4
• Identification du gène (NCBI): 9732
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers.

Publications

Species	Application	Title
human	WB,IF	Proc Natl Acad Sci U S A; Up-regulated cytotrophoblast DOCK4 contributes to over-invasion in placenta accreta spectrum.; Authors - Leah McNally
human	WB, IHC	Front Cell Dev Biol; USP36-Mediated Deubiquitination of DOCK4 Contributes to the Diabetic Renal Tubular Epithelial Cell Injury via Wnt/β-Catenin Signaling Pathway.; Authors - Suwei Zhu
human	WB	Front Oncol; miR-33b-3p Acts as a Tumor Suppressor by Targeting DOCK4 in Prostate Cancer.; Authors - Yu Mei

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