AXIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AXIN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 16541-1-AP

Synonyms

AXIN, axin 1, AXIN1, Axis inhibition protein 1, hAxin

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Validation Data Gallery

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  WB analysis of HT-1080 using 16541-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16541-1-AP (AXIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16541-1-AP (AXIN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16541-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16541-1-AP (AXIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HEK-293T cells, HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

16541-1-AP targets AXIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AXIN1 fusion protein Ag9858
• Full Name: axin 1
• Calculated Molecular Weight: 826aa,92 kDa; 862aa,95 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC044648
• Gene Symbol: AXIN1
• Gene ID (NCBI): 8312
• RRID: AB_2062409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).; Authors - Rui Zhang
human	WB	Front Oncol; Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.; Authors - Jinrong Zhu
human	WB	Cancer Gene Ther; Stabilizing and upregulating Axin with tankyrase inhibitor reverses 5-fluorouracil chemoresistance and proliferation by targeting the WNT/caveolin-1 axis in colorectal cancer cells.; Authors - Feng Luo
human	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue
human	WB	Exp Cell Res; The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.; Authors - Pan Wei
		FEBS Lett; Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression.; Authors - Yazhou Cui