Beta Catenin Monoclonal antibody

• Featured Product
• KD/KO Validated

Beta Catenin Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

76

CloneNo.

1B8A1

Cat no : 66379-1-Ig

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β-Catenin

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Validation Data Gallery

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  WB analysis using 66379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (beta-Catenin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 66379-1-Ig

  IP result of anti-beta-Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of mouse colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

• 

  IF Staining of human skin cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human skin cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66379-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66379-1-Ig (beta-Catenin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of MCF-7 using 66379-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse colon tissue, human skin cancer tissue, human liver cancer tissue, rat colon tissue, human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells, human liver cancer tissue, human skin cancer tissue
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:10000-1:40000
• Immunofluorescence (IF): IF : 1:750-1:3000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 61 publications below
• IHC: See 8 publications below
• IF: See 25 publications below
• IP: See 1 publications below
• CoIP: See 7 publications below

Product Information

66379-1-Ig targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat, sheep, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated molecular weight: 781 aa, 86 kDa
• Observed molecular weight: 92 kDa
• GenBank accession number: NM_001904
• Gene symbol: CTNNB1
• Gene ID (NCBI): 1499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	IF	Research (Wash D C); The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling; Authors - Xi Chen
human	IF	Acta Biomater; Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering; Authors - Zhen Liu
mouse	IF	Redox Biol; Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis.; Authors - Tirthankar Sinha
human	IHC	Oncogene; CRISPR/Cas9 library screening uncovered methylated PKP2 as a critical driver of lung cancer radioresistance by stabilizing β-catenin.; Authors - Chun Cheng ; KD Validated
human	WB,IF	Clin Transl Med; Targeting DCLK1 overcomes 5-fluorouracil resistance in colorectal cancer through inhibiting CCAR1/β-catenin pathway-mediated cancer stemness.; Authors - Lanqing Wang

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