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  • KD/KO Validated

PIP5K1A Polyclonal antibody

PIP5K1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15713-1-AP

Synonyms

PIP5K1-alpha, PIP5K1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, EC:2.7.1.68



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human liver tissue, human heart tissue, NCI-H1299 cells, PC-12 cells
Positive IP detected inHeLa cells, mouse heart tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15713-1-AP targets PIP5K1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5K1A fusion protein Ag8340
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated Molecular Weight 56 kDa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC007833
Gene Symbol PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2164696
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Protocols

Product Specific Protocols
WB protocol for PIP5K1A antibody 15713-1-APDownload protocol
IHC protocol for PIP5K1A antibody 15713-1-APDownload protocol
IF protocol for PIP5K1A antibody 15713-1-APDownload protocol
IP protocol for PIP5K1A antibody 15713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IF

Nat Cell Biol

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.

Authors - Rong Yueguang Y
humanWB

J Clin Invest

Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.

Authors - Jaerak Chang
humanWB

EMBO J

Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.

Authors - Takashi Baba
humanWB,IF,IP,IHC

Oncogene

The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.

Authors - Martuza Sarwar
  • KD Validated
mouseWB

Nutrients

Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice

Authors - Wenhui Xu
humanWB,IF

Front Cell Dev Biol

PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.

Authors - Tianyan Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million of cells during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody alone and in blue primary + secondary antibody

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 0,25µl/10^6cells
  • Cell Tissue Type: B cells
PIP5K1A Antibody Flow Cytometry validation (0,25µl/10^6cells dilution) in B cells (Cat no:15713-1-AP)