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PIP5K1A Polyclonal antibody
PIP5K1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15713-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, human liver tissue, human heart tissue, NCI-H1299 cells, PC-12 cells |
Positive IP detected in | HeLa cells, mouse heart tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 11 publications below |
IHC | See 2 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
Product Information
15713-1-AP targets PIP5K1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PIP5K1A fusion protein Ag8340 |
Full Name | phosphatidylinositol-4-phosphate 5-kinase, type I, alpha |
Calculated Molecular Weight | 56 kDa, 63 kDa |
Observed Molecular Weight | 63 kDa |
GenBank Accession Number | BC007833 |
Gene Symbol | PIP5K1A |
Gene ID (NCBI) | 8394 |
RRID | AB_2164696 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for PIP5K1A antibody 15713-1-AP | Download protocol |
IHC protocol for PIP5K1A antibody 15713-1-AP | Download protocol |
IF protocol for PIP5K1A antibody 15713-1-AP | Download protocol |
IP protocol for PIP5K1A antibody 15713-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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Nat Cell Biol Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation. | ||
J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. | ||
EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion. | ||
Oncogene The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.
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Nutrients Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice | ||
Front Cell Dev Biol PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Bastien (Verified Customer) (08-19-2020) | Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million of cells during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody alone and in blue primary + secondary antibody
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