Anticorps Polyclonal de lapin anti-Collagen Type VI

Collagen Type VI Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 14853-1-AP

Synonymes

COL6A2, Collagen alpha 2(VI) chain, Collagen Type VI, collagen, type VI, alpha 2, PP3610

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Galerie de données de validation

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  WB analysis using 14853-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14853-1-AP (Collagen Type VI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14853-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14853-1-AP (Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig skin using 14853-1-AP

  pig skin tissue were subjected to SDS PAGE followed by western blot with 14853-1-AP (Collagen Type VI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14853-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14853-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 14853-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14853-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14853-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14853-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14853-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14853-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, tissu cardiaque humain, tissu cutané de porc
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14853-1-AP cible Collagen Type VI dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type VI Protéine recombinante Ag6635
• Nom complet: collagen, type VI, alpha 2
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC065509
• Symbole du gène: COL6A2
• Identification du gène (NCBI): 1292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Collagen VI, composed of three polypeptides, alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains, is a beaded filament collagen found in most connective tissues. The alpha-1(VI) and alpha-2(VI) chains are 140 kDa, while the alpha-3(VI) chain is 200-300 kDa (PMID: 3792302, 3348212). COL6A2 gene encodes the alpha-2 subunit of type VI collagen. The alpha-2(VI) subunit has a calculated molecular weight of 109 kDa, and an apparent molecular weight of 140 kDa due to posttranslational modification. Mutations in COL6A2 gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Mol Cell Proteomics; Reductive Stress Selectively Disrupts Collagen Homeostasis and Modifies Growth Factor-independent Signaling Through the MAPK/Akt Pathway in Human Dermal Fibroblasts; Authors - Naomi A Carne
human	WB	FASEB J; Differentiation of diabetic foot ulcer-derived induced pluripotent stem cells reveals distinct cellular and tissue phenotypes.; Authors - Olga Kashpur
human	WB	Mol Cell Proteomics; Reductive Stress Selectively Disrupts Collagen Homeostasis and Modifies Growth Factor-independent Signaling Through the MAPK/Akt Pathway in Human Dermal Fibroblasts; Authors - Naomi A. Carne
human	IHC	Exp Ther Med; COL3A1, COL5A1 and COL6A2 serve as potential molecular biomarkers for osteoarthritis based on weighted gene co‑expression network analysis bioinformatics analysis; Authors - Yufeng Zhang
human	WB,IF	BMC Res Notes; Authentication of collagen VI antibodies.; Authors - Jamie Endicott

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (12-02-2019)

Excellent, no background

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

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