Collagen Type VI Polyclonal antibody

• Featured Product
• KD/KO Validated

Collagen Type VI Polyclonal Antibody for WB, IHC, IF, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (1)

Cat no : 17023-1-AP

Synonyms

COL6A1, COL6, OPLL, Collagen alpha-1(VI) chain, Collagen alpha 1(VI) chain

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Validation Data Gallery

• 

  WB analysis using 17023-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17023-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17023-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17023-1-AP (Collagen Type VI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 17023-1-AP

  IP result of anti-Collagen Type VI (IP:17023-1-AP, 4ug; Detection:17023-1-AP 1:2000) with mouse heart tissue lysate 1050 ug.

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17023-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17023-1-AP (Collagen Type VI antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 17023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Collagen Type VI antibody (17023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle tissue using 17023-1-AP

  IF result of anti-COL6A1 (17023-1-AP, 1:500) with PFA fixed mouse skeletal muscle tissue by Dr. Daniel Kopinke.

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Tested Applications

• Positive WB detected in: mouse heart tissue, human skeletal muscle tissue, human heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon tissue, human liver tissue, mouse liver tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon tissue
• Positive IF detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 9 publications below
• IF: See 8 publications below

Product Information

17023-1-AP targets Collagen Type VI in WB, IHC, IF, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag10288
• Full Name: collagen, type VI, alpha 1
• Calculated Molecular Weight: 1028 aa, 109 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC052575
• Gene Symbol: COL6A1
• Gene ID (NCBI): 1291
• RRID: AB_2229737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Publications

Species	Application	Title
mouse,rat	IF	Cell Death Differ; Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation; Authors - Guangjin Chen
zebrafish	WB,IF	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB,IHC	Oncogene; Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).; Authors - I García-Palmero
human	WB,IHC	Cancer Gene Ther; Schwann cell derived pleiotrophin stimulates fibroblast for proliferation and excessive collagen deposition in plexiform neurofibroma; Authors - Zhuowei Tian
human	WB	Oncotarget; Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth.; Authors - Yi-Ping Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Malak (Verified Customer) (02-24-2021)

It works well in the western blot

• Applications: Western Blot

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