LAMA4 (Isoform 3) Polyclonal antibody

LAMA4 (Isoform 3) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10465-1-AP

Synonyms

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4

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Validation Data Gallery

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  WB analysis of human heart using 10465-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10465-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10465-1-AP (LAMA4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 10465-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 10465-1-AP (LAMA4 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, human skeletal muscle tissue
• Positive IHC detected in: human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 6 publications below

Product Information

10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMA4 (Isoform 3) fusion protein Ag0736
• Full Name: laminin, alpha 4
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 11-13 kDa
• GenBank Accession Number: BC004241
• Gene Symbol: Laminin alpha 4/LAMA4
• Gene ID (NCBI): 3910
• RRID: AB_2234461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

Publications

Species	Application	Title
human	IHC	Arthritis Rheum; PMID: 21337318; Authors - Gutierrez-Roelens I I
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	IHC	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
human	IHC	Neuromuscul Disord; Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.; Authors - Doppler K K
human	IHC	J Neurosci Res; Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.; Authors - Xu Hongmin H
human	WB,IHC	Cancer Cell Int; MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.; Authors - Zhi-Xue Yang