Collagen Type II Polyclonal antibody

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  IHC staining of human lung using 15943-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15943-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 40x lens).

Collagen Type II Polyclonal Antibody for IHC, ELISA

Cat No. 15943-1-AP

Publications(63)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

IHC, IF, ELISA

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

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Tested Applications

• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 24 publications below
• IF: See 15 publications below

Product Information

15943-1-AP targets Collagen Type II in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8416

  Product name: Recombinant human COL2A1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1200-1487 aa of BC007252

  Sequence: GPPGNPGPPGPPGPPGPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL

• Full Name: collagen, type II, alpha 1
• Calculated Molecular Weight: 134 kDa
• GenBank Accession Number: BC007252
• Gene Symbol: COL2A1
• Gene ID (NCBI): 1280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02458
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Autophagy; HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.; Authors - Ruijun He
human	IHC	J Tissue Eng; High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration.; Authors - Tom Hodgkinson
human	IHC	Redox Biol; Advanced oxidation protein products increase TNF-α and IL-1β expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression.; Authors - Cong-Rui Liao
human		Oxid Med Cell Longev; KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2/ARE Signaling In Vitro and In Vivo.; Authors - Xiang Gao
mouse	IF	Oxid Med Cell Longev; Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration.; Authors - Xiao-Jun Yu
		Int J Mol Sci; Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.; Authors - Xuyang Sun