Beta Catenin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Beta Catenin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(975)

Rezensionen (3)

Kat-Nr. : 51067-2-AP

Synonyme

b cat, B-catenin, beta-Catenin, Catenin beta 1, Catenin beta-1

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  WB analysis of HeLa using 51067-2-AP

  WB result of Beta Catenin antibody (51067-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Catenin transfected HeLa cells.

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  WB analysis using 51067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51067-2-AP (Beta Catenin antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 51067-2-AP

  IP result of anti-Beta Catenin (IP:51067-2-AP, 4ug; Detection:51067-2-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IHC staining of mouse liver using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51067-2-AP (beta-Catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 51067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 51067-2-AP (Beta Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse epididymis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse epididymis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse epididymis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse epididymis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Beta Catenin antibody (51067-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 51067-2-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using 51067-2-AP

  Immunofluorescent analysis of HepG2 cells, using CTNNB1 antibody 51067-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 51067-2-AP (beta-Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of T-47D using 51067-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (51067-2-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 51067-2-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Beta Catenin (51067-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, C6-Zellen, HEK-293T-Zellen, HeLa-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe, mouse epididymis tissue
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, T-47D-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 850 publications below
• IHC: See 131 publications below
• IF: See 206 publications below
• IP: See 14 publications below
• CoIP: See 18 publications below
• ChIP: See 6 publications below

Produktinformation

51067-2-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA Beta Catenin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Hund, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Berechnetes Molekulargewicht: 781 aa, 86 kDa
• Beobachtetes Molekulargewicht: 92 kDa
• GenBank-Zugangsnummer: BC058926
• Gene symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publikationen

Species	Application	Title
mouse	WB,IF,IP,IHC	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IF	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Hepatology; Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.; Authors - Meng Lu
mouse,rat	WB	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
mouse	WB	Hepatology; Long noncoding RNA DANCR increases stemness features of hepatocellular carcinoma via de-repression of CTNNB1.; Authors - Sheng-Xian Yuan
zebrafish	IF	Nat Commun; ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.; Authors - Xiaoyun Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

kis (Verified Customer) (02-21-2025)

This antibody works well for western blotting at 1:1000 dilution for WB and 1:300 for IF.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1.1000

FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Bone Marrow MSCs

FH

Alzbeta (Verified Customer) (03-13-2019)

This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Primary rat neonatal cardiomyocytes

Beta Catenin Antibody Immunofluorescence, validation (1:100 dilution) in Primary rat neonatal cardiomyocytes (Cat no:51067-2-AP)