CSNK1A1 Polyclonal antibody

• 

  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CSNK1A1 antibody (55192-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CSNK1A1 antibody (55192-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

CSNK1A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55192-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CK1, CK1α, CKI alpha, CKI-alpha, CSNK

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, U-87 MG cells, rat brain tissue
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

55192-1-AP targets CSNK1A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: casein kinase 1, alpha 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 30-35 kDa, 42-45 kDa
• GenBank Accession Number: NM_001892
• Gene Symbol: CSNK1A1
• Gene ID (NCBI): 1452
• RRID: AB_11183034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48729
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu ; KD Validated
	WB	J Cell Biol; CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.; Authors - Xue Deng
human	IP	Virol Sin; CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells; Authors - Jing Xiong
mouse	WB,IF	Stem Cell Res Ther; Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.; Authors - Xiaomin Liu
human	WB	Int J Biol Sci; Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.; Authors - Yuyuan Zi
mouse	WB,IF	Endocrinology; Casein kinase 1α regulates testosterone synthesis and testis development in adult mice; Authors - Hongzhou Guo