Validation Data Gallery
|Positive WB detected in
|HeLa cells, MCF-7 cells, NIH/3T3 cells
|Positive IHC detected in
|human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55192-1-AP targets CSNK1A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|casein kinase 1, alpha 1
|Calculated molecular weight
|Observed molecular weight
|30-35 kDa, 42-45 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.
Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex
J Cell Biol
CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.
CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells
Stem Cell Res Ther
Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.
Int J Biol Sci
Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.
Casein kinase 1α regulates testosterone synthesis and testis development in adult mice