Alpha E-Catenin Polyclonal antibody

Alpha E-Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 12831-1-AP

Synonyms

alpha catenin, Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin

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Validation Data Gallery

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  WB analysis of mouse uterus using 12831-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12831-1-AP (Alpha E catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12831-1-AP

  IP Result of anti-Alpha E catenin (IP:12831-1-AP, 4ug; Detection:12831-1-AP 1:700) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 12831-1-AP

  Immunofluorescent analysis of A431 cells using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of MCF-7 using 12831-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Alpha E catenin antibody (12831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse uterus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach tissue, human colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IF: See 2 publications below

Product Information

12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha E-Catenin fusion protein Ag3565
• Full Name: catenin (cadherin-associated protein), alpha 1, 102kDa
• Calculated molecular weight: 906 aa, 100 kDa
• Observed molecular weight: 100 kDa
• GenBank accession number: BC031262
• Gene symbol: CTNNA1
• Gene ID (NCBI): 1495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publications

Species	Application	Title
human	WB	Helicobacter; Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.; Authors - Chen Zhang
human	WB	Cell Death Discov; Upregulation of CPNE7 in mesenchymal stromal cells promotes oral squamous cell carcinoma metastasis through the NF-κB pathway.; Authors - Xiaoli Ji
human	WB	Exp Ther Med; GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial-mesenchymal transition and metastasis through regulation of SLUG.; Authors - Yibin Zhou
human	WB	Balkan Med J; miR-509-3p Suppresses Migration, Invasion and Epithelial-Mesenchymal Transition in Melanoma Cells by Targeting Collagen Triple Helix Repeat Containing 1.; Authors - Keyu Yuan
human	WB	Med Oncol; Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines.; Authors - Yan Zhang
human		Oncol Rep; Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma.; Authors - Yanting Liu

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