Alpha E-Catenin Polyclonal antibody
Alpha E-Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12831-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse uterus tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human stomach tissue, human colon tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IF | See 2 publications below |
Product Information
12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Alpha E-Catenin fusion protein Ag3565 |
Full Name | catenin (cadherin-associated protein), alpha 1, 102kDa |
Calculated molecular weight | 906 aa, 100 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC031262 |
Gene symbol | CTNNA1 |
Gene ID (NCBI) | 1495 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.
Protocols
Product Specific Protocols | |
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WB protocol for Alpha E-Catenin antibody 12831-1-AP | Download protocol |
IHC protocol for Alpha E-Catenin antibody 12831-1-AP | Download protocol |
IF protocol for Alpha E-Catenin antibody 12831-1-AP | Download protocol |
IP protocol for Alpha E-Catenin antibody 12831-1-AP | Download protocol |
FC protocol for Alpha E-Catenin antibody 12831-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Helicobacter Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact. | ||
Cell Death Discov Upregulation of CPNE7 in mesenchymal stromal cells promotes oral squamous cell carcinoma metastasis through the NF-κB pathway. | ||
Exp Ther Med GATA4 is upregulated in nasopharyngeal cancer and facilitates epithelial-mesenchymal transition and metastasis through regulation of SLUG. | ||
Balkan Med J miR-509-3p Suppresses Migration, Invasion and Epithelial-Mesenchymal Transition in Melanoma Cells by Targeting Collagen Triple Helix Repeat Containing 1. | ||
Med Oncol Wnt signaling regulation of stem-like properties in human lung adenocarcinoma cell lines. | ||
Oncol Rep Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma. |