Alpha E-Catenin Polyclonal antibody

• 

  WB analysis of mouse uterus using 12831-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12831-1-AP (Alpha E catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12831-1-AP

  IP result of anti-Alpha E-Catenin (IP:12831-1-AP, 4ug; Detection:12831-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12831-1-AP (Alpha E-Catenin antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 12831-1-AP

  Immunofluorescent analysis of A431 cells using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Alpha E-Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12831-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CTNNA1, Renal carcinoma antigen NY-REN-13, Catenin alpha 1, CAP102, Alpha E catenin

---

Tested Applications

• Positive WB detected in: mouse uterus tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IF: See 3 publications below

Product Information

12831-1-AP targets Alpha E-Catenin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alpha E-Catenin fusion protein Ag3565
• Full Name: catenin (cadherin-associated protein), alpha 1, 102kDa
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC031262
• Gene Symbol: Alpha E-Catenin
• Gene ID (NCBI): 1495
• RRID: AB_2087822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35221
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publications

Species	Application	Title
human	WB	Oncogene; Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.; Authors - Q Zhou
mouse	IF	Elife; Selective YAP activation in Procr cells is essential for ovarian stem/progenitor expansion and epithelium repair.; Authors - Jingqiang Wang
human	WB	Oncogene; The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.; Authors - Quan Zhou
human	WB	Helicobacter; Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.; Authors - Chen Zhang
human	WB	Cells; P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer.; Authors - Nayden G Naydenov
human	IF	Sci Rep; SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth.; Authors - Rehab Alshargabi