Gamma Catenin Polyclonal antibody

Gamma Catenin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11146-1-AP

Synonyms

JUP, JUP/gamma Catenin, γ-Catenin, Desmoplakin-3, Desmoplakin III

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Validation Data Gallery

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  WB analysis of mouse heart using 11146-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11146-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11146-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 11146-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 11146-1-AP

  IP result of anti-Gamma Catenin (IP:11146-1-AP, 4ug; Detection:11146-1-AP 1:1000) with HT-29 cells lysate 1040 ug.

• 

  IHC staining of human pancreas cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bowen disease using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human skin using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 11146-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, HeLa cells, T-47D cells, A431 cells
• Positive IP detected in: HT-29 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, human colon cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11146-1-AP targets Gamma Catenin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Catenin fusion protein Ag1627
• Full Name: junction plakoglobin
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC011865
• Gene Symbol: Gamma Catenin
• Gene ID (NCBI): 3728
• RRID: AB_2265007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Publications

Species	Application	Title
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
mouse	WB	Oncogene; Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis.; Authors - M Shriver
human	WB	PLoS One; Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.; Authors - Bing Sun
mouse	WB	Biochem Biophys Res Commun; Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.; Authors - Yili Fang
mouse,rat	WB,IF,IP	Cell Rep; CYLD deubiquitinates plakoglobin to promote Cx43 membrane targeting and gap junction assembly in the heart; Authors - Wei Xie
human	IHC	Int J Mol Sci; Diagnostic and Prognostic Markers for Pancreatitis and Pancreatic Ductal Adenocarcinoma; Authors - Havish S Kantheti