VDAC3 Polyklonaler Antikörper

VDAC3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14451-1-AP

Synonyme

HD VDAC3, hVDAC3, VDAC 3, VDAC3

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Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 14451-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14451-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14451-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14451-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human osteosarcoma using 14451-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Osteosarkomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below

Produktinformation

14451-1-AP bindet in WB, IHC, ELISA VDAC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VDAC3 fusion protein Ag5960
• Vollständiger Name: voltage-dependent anion channel 3
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC056870
• Gene symbol: VDAC3
• Gene ID (NCBI): 7419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publikationen

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Mol Ther Nucleic Acids; Salvia miltiorrhiza-Derived Sal-miR-58 Induces Autophagy and Attenuates Inflammation in Vascular Smooth Muscle Cells.; Authors - Yan Qin
human	IHC	Oncotarget; Proteomics-based identification of VDAC1 as a tumor promoter in cervical carcinoma.; Authors - Changlin Zhang
	WB	J Cell Sci; Doublet 7 shortening, doublet 5-preferential poly-Glu reduction, and beating stall of sperm flagella in Ttll9-/- mice.; Authors - Alu Konno
rat	WB	Int J Mol Med; Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.; Authors - Lei Sun
human	WB	Front Aging Neurosci; The Protective Role of Mitochondrial Ferritin on Erastin-Induced Ferroptosis.; Authors - Yue-Qi Wang

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