TWIST1 Polyklonaler Antikörper

TWIST1 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 18125-1-AP

Synonyme

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1

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Galerie der Validierungsdaten

• 

  WB analysis of PC-3 using 18125-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 18125-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18125-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 18125-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18125-1-AP (TWIST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, MCF-7-Zellen, SGC-7901-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

18125-1-AP bindet in WB, IHC, IF, ELISA TWIST1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TWIST1 fusion protein Ag12700
• Vollständiger Name: twist homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 202 aa, 21 kDa
• Beobachtetes Molekulargewicht: 22 kDa, 28 kDa, 45-60 kDa
• GenBank-Zugangsnummer: BC036704
• Gene symbol: TWIST1
• Gene ID (NCBI): 7291
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publikationen

Species	Application	Title
human,mouse	IHC,IF	Theranostics; Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.; Authors - Limin Liu
human	WB	Int J Biochem Cell Biol; Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage.; Authors - Gege Yan
human	WB	Cancer Lett; Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry.; Authors - Changhui Yu
human	WB,IHC	Int J Cancer; Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.; Authors - Dejun Zhang
human	WB	Sci Rep; Correlations between TBL1XR1 and recurrence of colorectal cancer.; Authors - Hongda Liu
mouse	WB	Sci Rep; Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model.; Authors - Yu Liang

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