TWIST1-specific Polyclonal antibody

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  WB analysis using 25465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25465-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25465-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-453s using 25465-1-AP

  IP result of anti-TWIST1-specific (IP:25465-1-AP, 4ug; Detection:25465-1-AP 1:500) with MDA-MB-453s cells lysate 920ug.

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  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 25465-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 25465-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using TWIST1-specific antibody (25465-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25465-1-AP

Publications(180)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific

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Tested Applications

• Positive WB detected in: U-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells
• Positive IP detected in: MDA-MB-453s cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 166 publications below
• IHC: See 28 publications below
• IF: See 8 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• ChIP: See 5 publications below

Product Information

25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TWIST1-specific fusion protein Ag21924
• Full Name: twist homolog 1 (Drosophila)
• Calculated Molecular Weight: 202 aa, 21 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC036704
• Gene Symbol: TWIST1
• Gene ID (NCBI): 7291
• RRID: AB_2880093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15672
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition.; Authors - Yi Wu
mouse	WB,IHC	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
mouse	WB,IHC	Int J Biol Macromol; Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression; Authors - Yu-Wei Yen
human	WB,IHC	Int J Nanomedicine; Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model; Authors - Guoqiang Xu
human	IHC	Clin Transl Med; FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.; Authors - Lei Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pramod Bahadur (Verified Customer) (02-27-2024)

We are missing the vials so I dont have lot number.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PC3

TWIST1-specific Antibody Western Blot validation (1:1000 dilution) in PC3 (Cat no:25465-1-AP)