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TWIST1-specific Polyclonal antibody

TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 25465-1-AP

Synonyms

ACS3, bHLHa38, BPES2, BPES3, H twist, SCS, TWIST, twist homolog 1 (Drosophila), Twist related protein 1, TWIST1, TWIST1-specific



Tested Applications

Positive WB detected inU-251 cells, mouse brain tissue, Raji cells, A549 cells, MDA-MB-231 cells, PC-3 cells
Positive IP detected inMDA-MB-453s cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25465-1-AP is TWIST1-specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TWIST1-specific fusion protein Ag21924
Full Name twist homolog 1 (Drosophila)
Calculated molecular weight 202 aa, 21 kDa
Observed molecular weight25-30 kDa
GenBank accession numberBC036704
Gene symbol TWIST1
Gene ID (NCBI) 7291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Protocols

Product Specific Protocols
WB protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IHC protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IF protocol for TWIST1-specific antibody 25465-1-APDownload protocol
IP protocol for TWIST1-specific antibody 25465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Transl Med

FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression.

Authors - Lei Miao
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
humanWB

Cancer Lett

Mitochondrial oxidative stress by Lon-PYCR1 maintains an immunosuppressive tumor microenvironment that promotes cancer progression and metastasis.

Authors - Cheng-Liang Kuo
humanWB

Cancer Lett

UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer.

Authors - Dongying Wang
humanWB

Oncogene

The homeostasis-maintaining metabolites from bacterial stress response to bacteriophage infection suppress tumor metastasis.

Authors - Tianliang He
mouseWB

Arterioscler Thromb Vasc Biol

Embryologic Origin Influences Smooth Muscle Cell Phenotypic Modulation Signatures in Murine Marfan Syndrome Aortic Aneurysm

Authors - Albert J Pedroza