- Featured Product
- KD/KO Validated
TWIST1-specific Polyclonal antibody
TWIST1-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
147
Cat no : 25465-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, mouse brain tissue, Raji cells, SH-SY5Y cells, A549 cells, MDA-MB-231 cells, PC-3 cells |
Positive IP detected in | MDA-MB-453s cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 6 publications below |
WB | See 139 publications below |
IHC | See 16 publications below |
IF | See 7 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
ChIP | See 4 publications below |
Product Information
25465-1-AP targets TWIST1-specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TWIST1-specific fusion protein Ag21924 |
Full Name | twist homolog 1 (Drosophila) |
Calculated molecular weight | 202 aa, 21 kDa |
Observed molecular weight | 25-30 kDa |
GenBank accession number | BC036704 |
Gene symbol | TWIST1 |
Gene ID (NCBI) | 7291 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IHC protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IF protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
IP protocol for TWIST1-specific antibody 25465-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Adv Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition. | ||
Int J Biol Macromol Fucoidan/chitosan layered PLGA nanoparticles with melatonin loading for inducing intestinal absorption and addressing triple-negative breast cancer progression | ||
Clin Transl Med FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
Cancer Lett Mitochondrial oxidative stress by Lon-PYCR1 maintains an immunosuppressive tumor microenvironment that promotes cancer progression and metastasis. | ||
Cancer Lett UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer. |