TWIST2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TWIST2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11752-1-AP

Synonyms

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 11752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11752-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11752-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11752-1-AP (TWIST2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below

Product Information

11752-1-AP targets TWIST2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TWIST2 fusion protein Ag2348
• Full Name: twist homolog 2 (Drosophila)
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 21 kDa, 60 kDa
• GenBank Accession Number: BC033168
• Gene Symbol: TWIST2
• Gene ID (NCBI): 117581
• RRID: AB_2877791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

Publications

Species	Application	Title
human	IHC	Aging (Albany NY); LINC01235-TWIST2 feedback loop facilitates epithelial-mesenchymal transition in gastric cancer by inhibiting THBS2.; Authors - Yu-En Tan
human	WB	Front Cell Dev Biol; NR2F1-AS1/miR-190a/PHLDB2 Induces the Epithelial-Mesenchymal Transformation Process in Gastric Cancer by Promoting Phosphorylation of AKT3.; Authors - Jinqi Lv
human	WB	Front Oncol; TRIM50 Suppresses Pancreatic Cancer Progression and Reverses the Epithelial-Mesenchymal Transition via Facilitating the Ubiquitous Degradation of Snail1.; Authors - Rongkun Li
mouse	WB	Neoplasia; The Heterodimeric TWIST1-E12 Complex Drives the Oncogenic Potential of TWIST1 in Human Mammary Epithelial Cells.; Authors - Laurent Jacqueroud
mouse	WB	J Cancer; Asymmetric Division Gene Neurl2 Mediates Twist2 Regulation of Self-Renewal of Mouse Lewis Lung Cancer Stem Cells.; Authors - Doudou Liu ; KD Validated
human	WB	Gene; Overexpression of NuSAP1 is predictive of an unfavourable prognosis and promotes proliferation and invasion of triple-negative breast cancer cells via the Wnt/β-catenin/EMT signalling axis.; Authors - Li Sun