SNAI1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNAI1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

301

Reviews (1)

Cat no : 13099-1-AP

Synonyms

Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

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Validation Data Gallery

• 

  WB analysis of mouse heart using 13099-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13099-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13099-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 13099-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 13099-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13099-1-AP (SNAI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 13099-1-AP

  IP Result of anti-SNAI1 (IP:13099-1-AP, 4ug; Detection:13099-1-AP 1:600) with MCF-7 cells lysate 1040ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, PC-3 cells, BxPC-3 cells, human heart tissue, COLO 320 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 277 publications below
• IHC: See 29 publications below
• IF: See 26 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below

Product Information

13099-1-AP targets SNAI1 in WB, IP, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAI1 fusion protein Ag3723
• Full Name: snail homolog 1 (Drosophila)
• Calculated molecular weight: 264 aa, 29 kDa
• Observed molecular weight: 29-35 kDa
• GenBank accession number: BC012910
• Gene symbol: SNAI1
• Gene ID (NCBI): 6615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
human	WB	Autophagy; TGFB1-induced autophagy affects the pattern of pancreatic cancer progression in distinct ways depending on SMAD4 status.; Authors - Chen Liang
human	WB, ChIP	Cell Death Differ; Snail/PRMT5/NuRD complex contributes to DNA hypermethylation in cervical cancer by TET1 inhibition.; Authors - Jie Gao ; KD Validated
human	WB	Cancer Res; miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM.; Authors - Qiancheng Song
human	WB, IHC	Cancer Res; Raf kinase inhibitor protein RKIP enhances signaling by glycogen synthase kinase-3β.; Authors - Fahd Al-Mulla

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (11-23-2021)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2

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