TRPC5 Polyklonaler Antikörper

TRPC5 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 25890-1-AP

Synonyme

hTRP 5, hTRP5, Transient receptor protein 5, TRP 5, TRP5, TRPC5

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Galerie der Validierungsdaten

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  WB analysis using 25890-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25890-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25890-1-AP (TRPC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cerebellum using 25890-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25890-1-AP (TRPC5 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 25890-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 25890-1-AP (TRPC5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 3 publications below

Produktinformation

25890-1-AP bindet in WB, IHC, IF, ELISA TRPC5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPC5 fusion protein Ag23171
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 5
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC137271
• Gene symbol: TRPC5
• Gene ID (NCBI): 7224
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.

Publikationen

Species	Application	Title
human	WB	Cancer Lett; TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.; Authors - Da-Cen Lin
human	WB	Elife; Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.; Authors - Kangcheng Song
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
mouse	IF	Biochem Pharmacol; Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.; Authors - Cai Liang
human,mouse	WB	Biochem Biophys Res Commun; Trpc5-regulated AMPKα/mTOR autophagy pathway is associated with glucose metabolism disorders in low birth weight mice under overnutrition; Authors - Fei Zhou

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